Latest Resistance management for sustainable agriculture and improved public health

Test Method Library

The availability of standard, validated and easy-to-run methods for resistance detection in the world’s major insect pests is crucial for successful monitoring of resistance problems. Shown below are the IRAC Approved Test Methods which have been validated by IRAC member companies and collaborators to ensure that they provide reliable and reproducible data.

Most tests are specific to particular life-history stages and can only be used with confidence for toxicants which have been validated in the development of the methodology.  For more information please visit the IRAC Methods Team page.

Number Video Species Stage Methodology Modes of Action
000
  • Insect shipping guidelines .
  • All Stages of Development
  • Shipment
View Test Method 000
001 Has video
  • Green peach aphid Green peach aphid
  • All Stages of Development
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
View Test Method 001
002
  • Psyllids Psylla spp.
  • All Stages of Development
  • Dip
  • Organophosphates (1B)
View Test Method 002
003
  • European Red Mite European Red Mite
  • Twospotted Spider Mite Twospotted Spider Mite
  • Eggs
  • Dip
  • Clofentezine-Hexythiazox-Diflovidazin (10A)
View Test Method 003
004
  • Citrus red mite Citrus red mite
  • European Red Mite European Red Mite
  • Twospotted Spider Mite Twospotted Spider Mite
  • Adults
  • Dip
  • Carbamates (1A)
  • Organotin miticides (12B)
  • Propargite (12C)
View Test Method 004
005 Has video
  • Brown planthopper Brown planthopper
  • Rice green leafhopper Nephotettix cincticeps
  • Adults
  • Dip
View Test Method 005
006
  • Red flour beetle Red flour beetle
  • All Stages of Development
  • Filter-paper
  • Organophosphates (1B)
View Test Method 006
007
  • Corn earworm Corn earworm
  • Pseudoplusia includens
  • Tobacco budworm Tobacco budworm
  • Adults
  • Larvae
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Pyrethroids-Pyrethrins (3A)
  • Juvenile hormone analogues (7A)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
View Test Method 007
008
  • Tobacco Whitefly Tobacco Whitefly
  • Adults
  • Dip
  • Amitraz (19)
View Test Method 008
009
  • Pear Leaf Blister Moth Leucoptera scitella
  • Apple leaf miner moth Lithocolletis blancadella
  • Larvae
  • Eggs
  • Dip
  • Benzoylureas (15)
View Test Method 009
010 Has video
  • Western flower thrips Western flower thrips
  • Adults
  • Dip
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Pyrethroids-Pyrethrins (3A)
  • Spinosyns (5)
View Test Method 010
011 Has video
  • Pollen Beetle Pollen Beetle
  • Adults
  • Vial
  • Pyrethroids-Pyrethrins (3A)
View Test Method 011
012
  • European Red Mite European Red Mite
  • Adults
  • Petri-dish
  • METI acaricides and insecticides (21A)
View Test Method 012
013
  • European Red Mite European Red Mite
  • Adults
  • Dip
  • METI acaricides and insecticides (21A)
View Test Method 013
014
  • Western flower thrips Western flower thrips
  • Larvae
  • Dip
  • Benzoylureas (15)
View Test Method 014
015
  • Glasshouse whitefly Glasshouse whitefly
  • Tobacco Whitefly Tobacco Whitefly
  • Adults
  • Dip
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyridine azinomethine derivatives (9B)
  • Flonicamid (29)
View Test Method 015
016 Has video
  • Glasshouse whitefly Glasshouse whitefly
  • Tobacco Whitefly Tobacco Whitefly
  • Nymphs
  • Eggs
  • Dip
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyriproxyfen (7C)
  • Pyridine azinomethine derivatives (9B)
  • Buprofezin (16)
  • Tetronic and Tetramic acid derivatives (23)
View Test Method 016
017
  • Codling Moth Codling Moth
  • Larvae
  • Diet
  • Compounds of Unknown or Uncertain MoA (UN)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Spinosyns (5)
  • Avermectins-Milbemycins (6)
  • Fenoxycarb (7B)
  • Juvenile hormone analogues (7A)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
  • Indoxacarb (22A)
  • metaflumizone (22B)
  • Diamides (28)
View Test Method 017
018
  • Diamondback Moth Diamondback Moth
  • Larvae
  • Dip
  • Compounds of Unknown or Uncertain MoA (UN)
  • Carbamates (1A)
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Phenylpyrazoles (Fiproles) (2B)
  • Pyrethroids-Pyrethrins (3A)
  • Spinosyns (5)
  • Avermectins-Milbemycins (6)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
  • Indoxacarb (22A)
  • metaflumizone (22B)
  • Diamides (28)
View Test Method 018
019 Has video
  • Pea aphid Acyrthosiphon pisum
  • Black bean aphid Aphis fabae
  • Soybean aphid Aphis glycines
  • Buckthorn Aphid Aphis nasturtii
  • Foxglove aphid Aulacorthum solani
  • Currant-lettuce aphid Currant lettuce aphid
  • English grain aphid English grain aphid
  • Green peach aphid Green peach aphid
  • Potato Aphid Macrosiphum euphorbiae
  • Melon & Cotton Aphid Melon & Cotton Aphid
  • Rose-grain aphid Metopolophium dirhodum
  • Adults
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyridine azinomethine derivatives (9B)
  • Diafenthiuron (12A)
  • Tetronic and Tetramic acid derivatives (23)
  • Flonicamid (29)
View Test Method 019
020
  • Beet armyworm Beet armyworm
  • Corn earworm Corn earworm
  • Cotton leafworm Cotton leafworm
  • Fall armyworm Fall armyworm
  • Southern armyworm Spodoptera eridania
  • Cotton leafworm Spodoptera littoralis
  • Tobacco budworm Tobacco budworm
  • Larvae
  • Diet
  • Diamides (28)
View Test Method 020
021 Has video
  • Pollen Beetle Pollen Beetle
  • Adults
  • Vial
  • Neonicotinoids (4A)
View Test Method 021
022 Has video
  • Tomato leafminer Tomato leafminer
  • Dip
  • Spinosyns (5)
  • Indoxacarb (22A)
  • Diamides (28)
View Test Method 022
023
  • Green peach aphid Green peach aphid
  • Nymphs
  • Feeding
  • Diamides (28)
View Test Method 023
024
  • Melon & Cotton Aphid Melon & Cotton Aphid
  • Adults
  • Nymphs
  • Feeding
  • Diamides (28)
View Test Method 024
025 Has video
  • Pollen Beetle Pollen Beetle
  • Adults
  • Vial
  • Organophosphates (1B)
View Test Method 025
026
  • Housefly Housefly
  • Adults
  • Feeding
  • Neonicotinoids (4A)
View Test Method 026
027 Has video
  • Pollen Beetle Pollen Beetle
  • Adults
  • Vial
  • Indoxacarb (22A)
View Test Method 027
028
  • Brown marmorated stink bug Brown marmorated stink bug
  • Stink Bug Stink Bug
  • Adults
  • Dip
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 028
029
  • Brown marmorated stink bug Brown marmorated stink bug
  • Stink Bug Stink Bug
  • Adults
  • Topical
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 029
030 Has video
  • Brown marmorated stink bug Brown marmorated stink bug
  • Stink Bug Stink Bug
  • Adults
  • Vial
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 030
031
  • Cabbage stem flea beetle Cabbage stem flea beetle
  • Oilseed rape stem weevil Ceutorhynchus napi
  • Ceutorhynchus obstrictus
  • Cabbage leaf weevil Ceutorhynchus pallidactylus
  • Cabbage Flea Beetles Phyllotreta spp
  • Adults
  • Vial
  • Pyrethroids-Pyrethrins (3A)
View Test Method 031
032
  • Asian Citrus Psyllid
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Spinosyns (5)
  • Avermectins (6)
  • Diamides (28)
View Test Method 032
033
  • Western Tarnished Plant Bug Lygus hesperus
  • Adults
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Butenolides (4D)
  • Neonicotinoids (4A)
  • Nicotine (4B)
  • Sulfoximines (4C)
View Test Method 033
101
  • Southwestern Corn Borer Diatraea grandiosella
  • European Corn Borer European Corn Borer
  • Pink Borer Sesamia inferens
  • Sugarcane Borer
  • Larvae
  • Bacillus thuringiensis and the insecticidal proteins they produce (11A)
View Test Method 101

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