Resistance management for sustainable agriculture and improved public health

Test Methods

The availability of standard, validated and easy-to-run methods for resistance detection in the world’s major insect pests is crucial for successful monitoring of resistance problems. Shown below are the IRAC Approved Test Methods which have been validated by IRAC member companies and collaborators to ensure that they provide reliable and reproducible data.

Most tests are specific to particular life-history stages and can only be used with confidence for toxicants which have been validated in the development of the methodology.  For more information please visit the IRAC Methods Team page.

Number Video Species Stage Methodology Modes of Action
001 Has video
  • Green peach aphid Myzus persicae
  • All Stages of Development
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
View Test Method 001
002
  • Psyllids Psylla spp.
  • All Stages of Development
  • Dip
  • Organophosphates (1B)
View Test Method 002
003
  • European Red Mite Panonychus ulmi
  • Twospotted Spider Mite Tetranychus urticae
  • Eggs
  • Dip
  • Clofentezine-Hexythiazox-Diflovidazin (10A)
View Test Method 003
004
  • Citrus red spider mite Panonychus citri
  • European Red Mite Panonychus ulmi
  • Twospotted Spider Mite Tetranychus urticae
  • Adults
  • Dip
  • Carbamates (1A)
  • Organotin miticides (12B)
  • Propargite (12C)
View Test Method 004
005 Has video
  • Rice green leafhopper Nephotettix cincticeps
  • Brown planthopper Nilaparvata lugens
  • Adults
  • Dip
View Test Method 005
006
  • Red flour beetle Tribolium castaneum
  • All Stages of Development
  • Filter-paper
  • Organophosphates (1B)
View Test Method 006
007
  • Corn earworm Helicoverpa zea
  • Tobacco budworm Heliothis virescens
  • Pseudoplusia includens
  • Adults
  • Larvae
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Pyrethroids-Pyrethrins (3A)
  • Juvenile hormone analogues (7A)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
View Test Method 007
008
  • Tobacco Whitefly Bemisia tabaci
  • Adults
  • Dip
  • Amitraz (19)
View Test Method 008
009
  • Pear Leaf Blister Moth Leucoptera scitella
  • Apple leaf miner moth Lithocolletis blancaedella
  • Larvae
  • Eggs
  • Dip
  • Benzoylureas (15)
View Test Method 009
010 Has video
  • Western flower thrips Frankliniella occidentalis
  • Adults
  • Dip
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Pyrethroids-Pyrethrins (3A)
  • Spinosyns (5)
View Test Method 010
011 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
  • Pyrethroids-Pyrethrins (3A)
View Test Method 011
012
  • European Red Mite Panonychus ulmi
  • Adults
  • Petri-dish
  • METI acaricides and insecticides (21A)
View Test Method 012
013
  • European Red Mite Panonychus ulmi
  • Adults
  • Dip
  • METI acaricides and insecticides (21A)
View Test Method 013
014
  • Western flower thrips Frankliniella occidentalis
  • Larvae
  • Dip
  • Benzoylureas (15)
View Test Method 014
015
  • Tobacco Whitefly Bemisia tabaci
  • Glasshouse whitefly Trialeurodes vaporariorum
  • Adults
  • Dip
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyridine azinomethine derivatives (9B)
  • Flonicamid (29)
View Test Method 015
016 Has video
  • Tobacco Whitefly Bemisia tabaci
  • Glasshouse whitefly Trialeurodes vaporariorum
  • Nymphs
  • Eggs
  • Dip
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyriproxyfen (7C)
  • Pyridine azinomethine derivatives (9B)
  • Buprofezin (16)
  • Tetronic and Tetramic acid derivatives (23)
View Test Method 016
017
  • Codling Moth Cydia pomonella
  • Larvae
  • Diet
  • Compounds of Unknown or Uncertain MoA (UN)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Spinosyns (5)
  • Avermectins-Milbemycins (6)
  • Fenoxycarb (7B)
  • Juvenile hormone analogues (7A)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
  • Indoxacarb (22A)
  • metaflumizone (22B)
  • Diamides (28)
View Test Method 017
018
  • Diamondback Moth Plutella xylostella
  • Larvae
  • Dip
  • Compounds of Unknown or Uncertain MoA (UN)
  • Carbamates (1A)
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Phenylpyrazoles (Fiproles) (2B)
  • Pyrethroids-Pyrethrins (3A)
  • Spinosyns (5)
  • Avermectins-Milbemycins (6)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
  • Indoxacarb (22A)
  • metaflumizone (22B)
  • Diamides (28)
View Test Method 018
019 Has video
  • Pea aphid Acyrthosiphon pisum
  • Black bean aphid Aphis fabae
  • Soybean aphid Aphis glycines
  • Melon & Cotton Aphid Aphis gossypii
  • Buckthorn Aphid Aphis nasturtii
  • Foxglove aphid Aulacorthum solani
  • Potato Aphid Macrosiphum euphorbiae
  • Rose-grain aphid Metopolophium dirhodum
  • Green peach aphid Myzus persicae
  • Currant-lettuce aphid Nasonovia ribisnigri
  • English grain aphid Sitobion avenae
  • Adults
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyridine azinomethine derivatives (9B)
  • Diafenthiuron (12A)
  • Tetronic and Tetramic acid derivatives (23)
  • Flonicamid (29)
View Test Method 019
020
  • Corn earworm Helicoverpa zea
  • Tobacco budworm Heliothis virescens
  • Southern armyworm Spodoptera eridania
  • Beet armyworm Spodoptera exigua
  • Fall armyworm Spodoptera frugiperda
  • Cotton leafworm Spodoptera littoralis
  • Cotton leafworm Spodoptera litura
  • Larvae
  • Diet
  • Diamides (28)
View Test Method 020
021 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
  • Neonicotinoids (4A)
View Test Method 021
022 Has video
  • Tomato leafminer Tuta absoluta
  • Dip
  • Spinosyns (5)
  • Indoxacarb (22A)
  • Diamides (28)
View Test Method 022
023
  • Green peach aphid Myzus persicae
  • Nymphs
  • Feeding
  • Diamides (28)
View Test Method 023
024
  • Melon & Cotton Aphid Aphis gossypii
  • Adults
  • Nymphs
  • Feeding
  • Diamides (28)
View Test Method 024
025 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
  • Organophosphates (1B)
View Test Method 025
026
  • Housefly Musca domestica
  • Adults
  • Feeding
  • Neonicotinoids (4A)
View Test Method 026
027 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
  • Indoxacarb (22A)
View Test Method 027
028
  • Stink Bug Euschistus heros
  • Adults
  • Dip
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 028
029
  • Stink Bug Euschistus heros
  • Adults
  • Topical
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 029
030 Has video
  • Stink Bug Euschistus heros
  • Adults
  • Vial
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 030
031
  • Oilseed rape stem weevil Ceutorhynchus napi
  • Ceutorhynchus obstrictus
  • Cabbage leaf weevil Ceutorhynchus pallidactylus
  • Cabbage Flea Beetles Phyllotreta spp
  • Cabbage stem flea beetle Psylliodes chrysocephala
  • Adults
  • Vial
  • Pyrethroids-Pyrethrins (3A)
View Test Method 031
032
  • Asian Citrus Psyllid Diaphorina citri
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Spinosyns (5)
  • Avermectins (6)
  • Diamides (28)
View Test Method 032
033
  • Western Tarnished Plant Bug Lygus hesperus
  • Adults
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Butenolides (4D)
  • Neonicotinoids (4A)
  • Nicotine (4B)
  • Sulfoximines (4C)
View Test Method 033
101
  • Southwestern Corn Borer Diatraea grandiosella
  • Sugarcane Borer Diatraea saccharalis
  • European Corn Borer Ostrinia nubilalis
  • Pink Borer Sesamia inferens
  • Larvae
  • Bacillus thuringiensis and the insecticidal proteins they produce (11A)
View Test Method 101

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