Latest Resistance management for sustainable agriculture and improved public health

Test Method Library

The availability of standard, validated and easy-to-run methods for resistance detection in the world’s major insect pests is crucial for successful monitoring of resistance problems. Shown below are the IRAC Approved Test Methods which have been validated by IRAC member companies and collaborators to ensure that they provide reliable and reproducible data.

Most tests are specific to particular life-history stages and can only be used with confidence for toxicants which have been validated in the development of the methodology.  For more information please visit the IRAC Methods Team page.

Number Video Species Stage Methodology Modes of Action
000 Has video
  • All Stages of Development
  • Shipment
View Test Method 000
001 Has video
  • Green peach aphid Myzus persicae
  • All Stages of Development
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
View Test Method 001
002
  • Psyllids
  • All Stages of Development
  • Dip
  • Organophosphates (1B)
View Test Method 002
003
  • European Red Mite Panonychus ulmi
  • Twospotted Spider Mite Tetranychus urticae
  • Eggs
  • Dip
  • Clofentezine-Hexythiazox-Diflovidazin (10A)
View Test Method 003
004
  • Citrus red mite Panonychus citri
  • European Red Mite Panonychus ulmi
  • Twospotted Spider Mite Tetranychus urticae
  • Adults
  • Dip
  • Carbamates (1A)
  • Organotin miticides (12B)
  • Propargite (12C)
View Test Method 004
005 Has video
  • Brown planthopper Nilaparvata lugens
  • Rice green leafhopper
  • Adults
  • Dip
View Test Method 005
006
  • Red flour beetle Tribolium castaneum
  • All Stages of Development
  • Filter-paper
  • Organophosphates (1B)
View Test Method 006
007
  • Corn earworm Helicoverpa zea
  • Tobacco budworm Heliothis virescens
  • Adults
  • Larvae
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Pyrethroids-Pyrethrins (3A)
  • Juvenile hormone analogues (7A)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
View Test Method 007
008
  • Tobacco Whitefly Bemisia tabaci
  • Adults
  • Dip
  • Amitraz (19)
View Test Method 008
009
  • Pear Leaf Blister Moth
  • Apple leaf miner moth
  • Larvae
  • Eggs
  • Dip
  • Benzoylureas (15)
View Test Method 009
010 Has video
  • Western flower thrips Frankliniella occidentalis
  • Adults
  • Dip
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Pyrethroids-Pyrethrins (3A)
  • Spinosyns (5)
View Test Method 010
011 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
  • Pyrethroids-Pyrethrins (3A)
View Test Method 011
012
  • European Red Mite Panonychus ulmi
  • Adults
  • Petri-dish
  • METI acaricides and insecticides (21A)
View Test Method 012
013
  • European Red Mite Panonychus ulmi
  • Adults
  • Dip
  • METI acaricides and insecticides (21A)
View Test Method 013
014
  • Western flower thrips Frankliniella occidentalis
  • Larvae
  • Dip
  • Benzoylureas (15)
View Test Method 014
015
  • Glasshouse whitefly Trialeurodes vaporariorum
  • Tobacco Whitefly Bemisia tabaci
  • Adults
  • Dip
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyridine azinomethine derivatives (9B)
  • Flonicamid (29)
View Test Method 015
016 Has video
  • Glasshouse whitefly Trialeurodes vaporariorum
  • Tobacco Whitefly Bemisia tabaci
  • Nymphs
  • Eggs
  • Dip
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyriproxyfen (7C)
  • Pyridine azinomethine derivatives (9B)
  • Buprofezin (16)
  • Tetronic and Tetramic acid derivatives (23)
View Test Method 016
017
  • Codling Moth Cydia pomonella
  • Larvae
  • Diet
  • Compounds of Unknown or Uncertain MoA (UN)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Spinosyns (5)
  • Avermectins-Milbemycins (6)
  • Fenoxycarb (7B)
  • Juvenile hormone analogues (7A)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
  • Indoxacarb (22A)
  • metaflumizone (22B)
  • Diamides (28)
View Test Method 017
018
  • Diamondback Moth Plutella xylostella
  • Larvae
  • Dip
  • Compounds of Unknown or Uncertain MoA (UN)
  • Carbamates (1A)
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Phenylpyrazoles (Fiproles) (2B)
  • Pyrethroids-Pyrethrins (3A)
  • Spinosyns (5)
  • Avermectins-Milbemycins (6)
  • Benzoylureas (15)
  • Diacylhydrazines (18)
  • Indoxacarb (22A)
  • metaflumizone (22B)
  • Diamides (28)
View Test Method 018
019 Has video
  • Pea aphid
  • Black bean aphid
  • Soybean aphid
  • Buckthorn Aphid
  • Foxglove aphid
  • Currant-lettuce aphid Nasonovia ribisnigri
  • English grain aphid Sitobion avenae
  • Green peach aphid Myzus persicae
  • Potato Aphid
  • Melon & Cotton Aphid Aphis gossypii
  • Rose-grain aphid
  • Adults
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Pyridine azinomethine derivatives (9B)
  • Diafenthiuron (12A)
  • Tetronic and Tetramic acid derivatives (23)
  • Flonicamid (29)
View Test Method 019
020 Has video
  • Beet armyworm Spodoptera exigua
  • Corn earworm Helicoverpa zea
  • Fall armyworm Spodoptera frugiperda
  • Old world bollworm Helicoverpa armigera
  • Southern armyworm
  • Cotton leafworm
  • Taro caterpillar Spodoptera litura
  • Tobacco budworm Heliothis virescens
  • Larvae
  • Diet
  • Diamides (28)
View Test Method 020
021 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
  • Neonicotinoids (4A)
View Test Method 021
022 Has video
  • Tomato leafminer Tuta absoluta
  • Dip
  • Spinosyns (5)
  • Indoxacarb (22A)
  • Diamides (28)
View Test Method 022
023
  • Green peach aphid Myzus persicae
  • Nymphs
  • Feeding
  • Diamides (28)
View Test Method 023
024
  • Melon & Cotton Aphid Aphis gossypii
  • Adults
  • Nymphs
  • Feeding
  • Diamides (28)
View Test Method 024
025 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
  • Organophosphates (1B)
View Test Method 025
026
  • Housefly Musca domestica
  • Adults
  • Feeding
  • Neonicotinoids (4A)
View Test Method 026
027 Has video
  • Pollen Beetle Meligethes aeneus
  • Adults
  • Vial
  • Indoxacarb (22A)
View Test Method 027
028
  • Brown marmorated stink bug Halyomorpha halys
  • Stink Bug Euschistus heros
  • Adults
  • Dip
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 028
029
  • Brown marmorated stink bug Halyomorpha halys
  • Stink Bug Euschistus heros
  • Adults
  • Topical
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 029
030 Has video
  • Brown marmorated stink bug Halyomorpha halys
  • Stink Bug Euschistus heros
  • Adults
  • Vial
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
View Test Method 030
031
  • Cabbage stem flea beetle Psylliodes chrysocephala
  • Oilseed rape stem weevil
  • Cabbage leaf weevil
  • Cabbage Flea Beetles
  • Adults
  • Vial
  • Pyrethroids-Pyrethrins (3A)
View Test Method 031
032
  • Diaphorina citri
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Neonicotinoids (4A)
  • Spinosyns (5)
  • Avermectins (6)
  • Diamides (28)
View Test Method 032
033
  • Western Tarnished Plant Bug
  • Adults
  • Nymphs
  • Dip
  • Carbamates (1A)
  • Organophosphates (1B)
  • Pyrethroids-Pyrethrins (3A)
  • Butenolides (4D)
  • Neonicotinoids (4A)
  • Nicotine (4B)
  • Sulfoximines (4C)
View Test Method 033
034
  • Rice stem borer Chilo suppressalis
  • Yellow Stem Borer Scirpophaga incertulas
  • Pink Borer
  • Larvae
  • Dip
  • Indoxacarb (22A)
  • Diamides (28)
View Test Method 034
035
  • Cotton leafhopper Amrasca biguttula biguttula
  • Nymphs
  • Dip
  • Organophosphates (1B)
  • Pyropenes (9D)
  • Flonicamid (29)
View Test Method 035
036
  • Bed Bugs Cimex lectularius
  • Adults
  • Nymphs
  • Topical
  • Carbamates (1A)
  • Cyclodiene organochlorines (2A)
  • Phenylpyrazoles (Fiproles) (2B)
  • Pyrethroids-Pyrethrins (3A)
  • Nicotinic acetylcholine receptor (nAChR) competitive modulators (4)
  • Oxadiazines (22A)
  • semicarbazones (22B)
View Test Method 036
037
  • German Cockroach
  • Cockroaches Cockroach Species
  • Adults
  • Nymphs
  • Topical
  • Carbamates (1A)
  • Organophosphates (1B)
  • Cyclodiene organochlorines (2A)
  • Phenylpyrazoles (Fiproles) (2B)
  • Pyrethroids-Pyrethrins (3A)
  • Nicotinic acetylcholine receptor (nAChR) competitive modulators (4)
  • Oxadiazines (22A)
  • semicarbazones (22B)
View Test Method 037
101
  • Southwestern Corn Borer
  • European Corn Borer Ostrinia nubilalis
  • Pink Borer
  • Diatraea saccharalis
  • Larvae
  • Bacillus thuringiensis and the insecticidal proteins they produce (11A)
View Test Method 101

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